BRCA2 Knockout cell line (LNCaP Clone FGC)

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location： Home > Products > KO Cell Lines > BRCA2 Knockout cell line (LNCaP Clone FGC)

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BRCA2 Knockout cell line (LNCaP Clone FGC) Order

BRCA2 Knockout cell line (LNCaP Clone FGC)
Catalog#
YKO-H1090
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
BRCA2 Knockout cell line (LNCaP Clone FGC)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
BRCA2 Knockout cell line (LNCaP Clone FGC)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
BRCA2
Gene ID
675
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%RPMI-1640+10% FBS+1%Sodium pyruvate+1%NEAA
Accession
CVCL_E0XF(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript BRCA2-201)
Validation: PCR+Sanger Sequencing
BRCA2 Knockout cell line (LNCaP Clone FGC) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:LNCaP Clone FGC		 STR Info (Cell bank)
Cell Line:LNCAP
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 10121012
D7S820 9.110.39.110.3
D16S539 11111111
VWA 16181618
TH01 9999
AMEL XYXY
TPOX 8989
CSF1PO 10111011
D12S391 2122
FGA 1920
D2S1338 1616
D21S11 2932.2
D18S51 1112
D8S1179 121314.0
D3S1358 1516
D6S1043 111218.0
PENTAE 1216
D19S433 13.215
PENTAD 1212
D1S1656 1616
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BRCA2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the LNCaP Clone FGC cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BRCA2 gene knockout BRCA2 Knockout cell line (LNCaP Clone FGC) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
BRCA2 Knockout cell line (LNCaP Clone FGC)
BRCA2 Knockout cell line (LNCaP Clone FGC)
Catalog#
YKO-H1090
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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